Tottenham midfielder Winks has undergone groin surgery and faces a race to be fit for the tournament and also the Champions League final on June 1.
Winks has not played since April 9 but his inclusion shows just how important he is to Southgate’s England plans.
But Southgate is banking on Winks and his Tottenham team mate Harry Kane to be fit as they step up their recovery.
Southampton’s Nathan Redmond also gets a recall while James Ward-Prowse and Fabian Delph are in after Chelsea’s Ruben Loftus-Cheek got an ankle injury on his club’s end of season tour to Boston.
Manchester United player of the season, Luke Shaw, has been left out, with Ben Chilwell and Danny Rose preferred.
Borussia Dortmund’s Jadon Sancho retains his place in attack, as does Bournemouth’s Callum Wilson.
Nine of the 27 players named will feature in the Champions League final just five days beforehand.
Full England squad
Goalkeepers: Pickford, Heaton, Butland
Defenders: Gomez, Alexander-Arnold, Maguire, Chilwell, Keane, Rose, Walker, Stones, Trippier
Midfielders: Barkley, Alli, Dier, Henderson, Winks, Rice, Ward-Powse, Delph
Forwards: Kane, Sterling, Lingard, Rashford, Sancho, Wilson, Redmond
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